Free radicals are highly reactive chemical species that contain an unpaired electron. These radicals are unstable and are constantly “searching” to combine with another molecule to achieve a more stable configuration (McBride and Kraemer, 1999; Sen, 1995). When certain molecules are oxidized by free radicals their function is impaired. Reactive oxygen species (ROS) are the key radicals in biological systems. They include the superoxide anion, hydroxyl, alkoxyl, and peroxyl radical groups (Cooper, Vollaard, Choueiri, and Wilson, 2002; McBride and Kraemer, 1999; Sen, 1995). Hydrogen peroxide is not an oxygen radical; however, it is considered in the ROS family because of its ability to generate the hydroxyl radical (Clarkson and Thompson, 2000).
Metabolic stress during high intensity, moderate to long distance exercise (Cooper, et al., 2002) and mechanical stress such as repeated lengthening (eccentric) muscle contractions (i.e. downhill running) have been shown to result in the generation of ROS (Kendall and Eston, 2002; McBride and Kraemer, 1999).