Calorie availability pivotally affects metabolic rate, reproductive fitness, growth, and survival in fish (Tocher, 2003). Calories in the form of fatty acids are the most significant source of ATP for many species of fish. Accordingly, fish manipulate storage and mobilization of fatty acids as part of their natural history resulting in a variety of outcomes. Striped bass increase intracellular lipid droplets 13-fold in red muscle during cold acclimation (Egginton and Sidell, 1989) and salmon increase serum cholesterol without eating during spawning migrations (Farrell and Munt, 1983).
The expression of fat metabolism genes is regulated by free long chain fatty acids and their metabolic by-products, however the definitive mechanism by which they do so remains elusive (Duplus et al., 2000). Characterizing the molecular signaling behind these changes in fat metabolism has traditionally been approached by looking at candidate proteins, (e.g. fatty acid binding proteins (FABPs) (Londraville and Sidell, 1995)) organelle function (peroxisomes (Crockett and Sidell, 1993)), and enzymatic indicators of fatty acid flux (Sidell et al., 1995). However, these are specific indicators, not an integrated global mechanism. One family of transcription factors, the peroxisome proliferated-activated receptors (PPARs), has the potential to attach a mechanism to the metabolic changes detailed above.